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Término solicitado: MEDRANO, ANAHI/(22)

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Tipo de Docum.: artículo
Título: Electrochemical determination of β-Lactoglobulin employing a polystyrene bead-modified carbon nanotube ink
Autor: Molinari, Judith; Florez, Laura; Medrano, Anahí; Monsalve, Leandro; Ybarra, Gabriel
Título Ser./Col.: Biosensors, 8(4)
Autor Inst. Ser./Col.: Multidisciplinary Digital Publishing Institute. MDPI. Basilea. CH
Idioma: eng
Datos de Edición: s.l. MDPI. 2018.
Pág./Vol.: 9p.
Unidad técnica: INTI-Procesos Superficiales; INTI-Micro y Nanoelectrónica.
Descriptores: Métodos electroquímicos; Sensores electroquímicos; Nanotubos de carbón; Alérgenos; Alimentos alergénicos; Poliestireno; Tintas; Biosensores
Resumen: In this article, we introduce the use of a carboxy-functionalized waterborne carbon nanotube ink for the fabrication of an amperometric biosensor aimed at the quantification of β-lactoglobulin. Detection of this protein from cow’s milk was performed by a sandwich immunoassay onto printed carbon nanotube electrodes. The electrodes were printed using a carbon nanotube ink modified with polystyrene beads containing a high amount of carboxylic groups for protein immobilization. This strategy showed enhanced sensing performance compared to the use of oxidative treatments for the functionalization of electrodes. These electrodes showed an excellent electrochemical behavior, and proteins could be immobilized on their surface via the carbodiimide reaction. These antibody-immobilized carbon nanotube electrodes allowed for the detection of β-lactoglobulin in sub-ppm concentrations.

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https://www.mdpi.com/2079-6374/8/4/109



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