Término solicitado: ALBERTO, EDGARDO/(22)
2 Registro/s encontrado/s en CAT+NORMA
| Tipo de Docum.: | artículo |
| Título: | Functional evaluation of ToxA promoter in Trichoderma reesei Rut- C30 |
| Autor: | Ortiz, Gastón E.; Blasco, Martín; Albertó, Edgardo |
| Título Ser./Col.: | BioTechnology : an Indian Journal, 12(1) |
| Autor Inst. Ser./Col.: | Trade Science Inc. |
| Idioma: | eng |
| Datos de Edición: | s.l. Trade Science Inc. 2016. |
| Pág./Vol.: | p. 39-44. |
| Unidad técnica: | INTI-Biotecnología Industrial. |
| Descriptores: | Proteínas; Fluorimetría; Hongos; Evaluación; Biotecnología; Análisis microbiológico |
| Resumen: | In order to expand the collection of promoters studied in Trichoderma reesei Rut-C30, a functionalstudy of the ToxA promoter was carried out by analysis of GFP expression in T. reesei Rut-C30. For this purpose, the binary pCBCT expression vector was employed to transform T.reesei Rut- C30. The transformants obtained were evaluated by means of fluorescence microscopy, fluorometry, dot blot and Western blot analysis. The low levels of cytoplasmic GFP protein in fungal hyphae suggest that ToxA promoter works as a weak constitutive promoter which can drive successfully the expression of heterologous proteins in T. reesei Rut-C30 |
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Trabajo de INTI |
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URL: |
https://www.tsijournals.com/articles/functional-evaluation-of-toxa-promoter-in-trichoderma-reeseirutc30.pdf |
| Tipo de Docum.: | artículo |
| Título: | An economical and high throughput alternative for endoglucanase activity determination |
| Autor: | Ortiz, Gastón E.; Blasco, Martín; Albertó, Edgardo |
| Título Ser./Col.: | BioTechnology : an Indian Journal, 12(2) |
| Autor Inst. Ser./Col.: | Trade Science Inc. |
| Idioma: | eng |
| Datos de Edición: | s.l. Trade Science Inc. 2016. |
| Pág./Vol.: | p. 70-74. |
| Unidad técnica: | INTI-Biotecnología Industrial. |
| Descriptores: | Celulasa; Ensayos biológicos; Enzimas; Análisis enzimático; Biotecnología; Microbiología |
| Resumen: | The search for new strains with good productivity of cellulolytic enzymes is always necessary to improve the processes are usually employed. Screening protocols used for these purposes require a low cost and high throughput assay for determination of endoglucanase activity. However, the test proposed by the IUPAC and other microadaptations not meet this requirement. The aim of our work is to obtain an economical and high- performance proposed by IUPAC for determination of endoglucanase activity assay adaptation. For this we reduced the assay volume and investigate the effect of this reduction on endoglucanase activity determination.As a result a miniaturized assay statistically correlated with the assay proposed by IUPAC reduced 10-fold lower scale was obtained. As a result a new economic and high performance adaptation for endoglucanase activity assay based on the IUPAC protocol was obtained. |
| Ver Documento
| |
Trabajo de INTI |
|
URL: |
https://www.tsijournals.com/articles/an-economical-and-high-throughput-alternative-for-endoglucanase-activity-determination.pdf |
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